Reverse transcriptase PCR: RT-PCR involved mRNA since the commencing material and it employs reverse transcriptase to transform mRNA into the complementary DNA (cDNA). This cDNA is then amplified with the help of regular PCR. Touchdown PCR: Annealing temperature through the first two cycles of amplification is set at about three–ten°C https://www.facebook.com/NewTechFind/posts/pfbid02RncFQX8CkjAPpZAaHcChBuCRzeG2YdMLeWZbJ9C4o1fi9ZzNf6Lyqa9d8ohEWRjsl