Normally this strain is 0.25 inches of h2o column, but might be elevated or decreased as the method calls for. Isolators need to be periodically leak tested to make sure their integrity and prevent escape of the decontamination agent. Leak testing can be done by several techniques such as https://www.tumblr.com/tailinscitech/805881091208724480/applications-and-benefits-of-tailin-containment?source=share